Plasma alkaline phosphatase isozymes: Isolation and characterization of isozymes
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Plasma alkaline phosphatase isozymes: isolation and characterization of isozymes.
I. The two isozymes of alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) detectable in human blood group O plasma have been separated from one another and isolated in a high state of purity. With p-nitrophenyl phosphate as substrate, the catalytic properties of the two isozymes appear to be the same with respect to pH optimum, lO.5; Kin, o.35-o.4 mM, and activity in ...
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A large number of studies on a lkaline phosphatase (AP) of chicken plasma have been made during the past forty years. Most of those studies are on the enzymic activity and the polymorphic studies are relatively few . In most of the polymorphic studies of chicken plasma AP, a starch gel electrophoresis has been employed. Major results by other investigators'"' may be summarized as follows. AP co...
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The three human isozymes of alkaline phosphatases were quantitatively determined in normal testis and seminoma tissues. The highly selective assays were based on isozyme specific monoclonal antibodies. In the normal testis approximately 90% of the catalytic activity originates from the tissue unspecific alkaline phosphatase, and the remaining activity was due to trace expression of both intesti...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Enzymology
سال: 1971
ISSN: 0005-2744
DOI: 10.1016/0005-2744(71)90092-1